![]() Call 01698 338844 should you require a visit from one of our team. Please don’t hesitate to call us if we can be of assistance.We now have a network of seven sales representatives throughout the UK who are capable of providing full technical support on all the products listed. We would like to thank you for taking time to consider us for your valued business. Remember when you’re happy, we’re delighted! We invite you to test our commitment at all times. In other words, we feel the track record of the people and the products at Thistle will stand up to the challenges that lie ahead. We believe our customers are our most valuable assets” Satisfied customers normally maintain a long term purchasing relationship and this, in turn, ensures we know how to respond to requests at all times. “Reliable products give trouble free use. Our philosophy is therefore straightforward: The staff at Thistle Scientific Ltd., are determined to offer and maintain the highest of standards in terms of customer care. Asteria Single-cell RNA-seq Benchtop Kit.SpinVessel® – Mixing without stir bars.End-point Multiplex and GC-rich PCR mixes.The Trans-Blot cell accommodates three gel holder cassettes, each with a 16 x 20 cm blotting area. Chemiluminescence Documentation Systems Trans-Blot Cell The Trans-Blot cell offers a choice of plate or wire electrodes and variable placement of the electrodes for either standard or high-intensity blotting options.Mix the Clarity Western ECL Substrate Kit components in a 1:1 ratio. It is important to use an ECL substrate that has good sensitivity and long signal duration, such as the Clarity Western ECL Substrate. After the final wash step, keep the blot in TBST while preparing for blot detectionĪll PrecisionAb Antibodies were validated using enhanced chemiluminescent (ECL) detection. Rinse the blot with 15 ml TBST at RT for 5 min. Incubate the blot in the secondary antibody and blocking buffer solution at RT for 1 hr with gentle agitation The Bio-Rad Mini Protean Tetra Cell is a vertical polyacrylamide gel electrophoresis system that provides rapid, high resolution separation of protein. Please refer to the antibody product page for details on the exact secondary antibody used during the validation process. Repeat for a total of five washesĭilute the appropriate secondary antibody in 10 ml blocking buffer according to the following table: Incubate the blot in the primary antibody and blocking buffer solution at 4☌ overnight with gentle agitation Please see the validation protocol (bulletin 6603) for more details.ĭilute the primary antibody 1:1,000 in 10 ml blocking buffer ![]() If using BSA, you may notice some nonspecific bands due to its low stringency. We recommend using casein or nonfat dried milk for blocking. When using casein, do not block for longer than 30 min to prevent reduction in signal specificity. Load the control cell lysate adjacent to your samples and the molecular weight (MW) marker (see diagram).ĭuring the validation process, we blocked for 30 min at room temperature (RT) in blocking buffer + 0.1% Tween 20. If using BME, add 180 μl H20, 200 μl 2x Laemmli Sample Buffer, and 20 μl BME If using DTT, add 190 μl H20, 200 μl 2x Laemmli Sample Buffer, and 10 μl 2 M DTT Reconstitute 400 µg lysate in one of the following ways, depending on the reducing reagent used: Secondary antibodies (see antibody datasheet)
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